Antidiarrhoeal potentials of methanol bark extract of Hymenocardia Acida Tul (Euphorbiaceae) in laboratory animals

Abstract Background The plant Hymenocardia acida (Euphorbiaceae) is utilized as herbal preparation against diarrhoea, dysentery and other diseases. We aimed to determine the antidiarrhoeal potentials of (MEHA) stem bark in vivo vitro. Preliminary phytochemical contents, well acute toxicity effect extract, were investigated based on standard experimental methods. properties MEHA at 150, 300 600 mg/kg studied diarrhoea induced by castor oil, intestinal fluid accumulation, movement tests using distilled water (10 ml/kg) loperamide/atropine sulphate control groups. Besides, vitro effects extract (8 × 10 −2 –640 mg/ml) rabbit jejunum guinea-pig ileum evaluated. Results Phytochemical screening showed alkaloids, glycoside, saponins, tannins, triterpenes, flavonoids steroids MEHA. median lethal dose (LD 50 ) after oral administration was approximately greater than 2000 mg/kg. declined onset remarkably decreased number watery stools group that received It also elicited a remarkable non-dose-dependent reduction volume. At 1000 mg/kg, significantly inhibited charcoal movement. In addition, decrease contractility over time relaxed guinea pig ileum. it concentration-dependent attenuation acetylcholine histamine-induced contraction. Conclusion under investigation revealed promising justified its traditional claim for use diarrhoea.